pcag-ert2-cre-ert2  (Addgene inc)

Journal: PLoS ONE

Article Title: miR-132 Enhances Dendritic Morphogenesis, Spine Density, Synaptic Integration, and Survival of Newborn Olfactory Bulb Neurons

doi: 10.1371/journal.pone.0038174

Figure Lengend Snippet: ( A ) qRT-PCR of miR-132 fold-change normalized to control RNA U6 from the ipsilateral bulbs containing pSico 132 -expressing neurons (red) and from the contralateral bulbs (black) at 5 weeks post-tamoxifen (wpt) injections given 2 wpe (N = 5 mice each). ( B and C ) Plots of the summed dendrite length (B) and bar graphs of the total dendritic length (C) of pSico SCR (n = 47) and pSico 132 -containing neurons (n = 51) at 5 wpt injections given at 7 dpe (i.e. 6 wpe). ( D ) Bar graphs of the frequency of GABAergic PSCs in pSico SCR - and pSico 132 -containing neurons at 5 wpt (n = 13 black and 15 red, respectively). ( G ) Sample images illustrating the density of pSico SCR and pSico 132 neurons in OB coronal sections. ( H and I ) Bar graphs of absolute (H) and normalized (I) RFP + ( i.e. pSico SCR , black and pSico 132 , red) neuron density in the GCL (N = 8 and 9 mice, 3–4 images per mouse, respectively). Scale bar: 100 µm.

Article Snippet: The pSico plasmids were co-injected with the tamoxifen-inducible Cre-recombinase expression vector pCAG- ERT2 Cre ERT2 (Addgene, C. Cepko) as well as a pCAG-tdTomato (noted RFP) vector that was constructed using the pCMV-tdTomato vector from Clontech.

Techniques: Quantitative RT-PCR, Expressing